Identification of Sand Flies (Diptera: Psychodidae) Collected from Cutaneous Leishmaniasis Endemic Focus in the  Ho Municipality, Ghana

Edna Dzifa Doe; Godwin Kwakye-Nuako; Seth Offei Addo; Alexander Egyir-Yawson

doi:10.21467/ias.10.1.33-44

Authors

Edna Dzifa Doe Cellular and Clinical Research Centre, Radiological and Medical Sciences Research Institute, GAEC, Ghana https://orcid.org/0000-0002-0956-2830
Godwin Kwakye-Nuako Department of Biomedical Sciences, School of Allied Health Sciences, University of Cape Coast, Ghana http://orcid.org/0000-0002-1865-1261
Seth Offei Addo Noguchi Memorial Institute for Medical Research, Parasitology Department, University of Ghana, Legon https://orcid.org/0000-0003-4470-4504
Alexander Egyir-Yawson Department of Biomedical Sciences, School of Allied Health Sciences, University of Cape Coast, Ghana http://orcid.org/0000-0002-4447-4027

DOI:

https://doi.org/10.21467/ias.10.1.33-44

Abstract

Leishmaniasis, is a vector-borne disease transmitted to humans through the bite of infected female sand flies. Active and continuous monitoring of the sand fly is an important aspect of disease control. Thus, the correct identification of its vectors is paramount in this regard. Objective: The study was conducted to morphologically and molecularly identify female sand fly species in a cutaneous leishmaniasis endemic focus collected in three villages in the Ho Municipality of the Volta region. CDC light traps and sticky paper traps was used for the collection of the sand flies. The morphologically identified sand flies was molecularly confirmed by running PCR with the mitochondrial cytochrome c oxidase gene subunit I (COI) primers and DNA sequenced. A total of 537 sand flies was collected, made up of 363 females and 174 males. Eleven different species of sand flies was morphologically identified – one Phlebotomus species and ten Sergentomyia species. The PCR amplified products showed bands of molecular weights 658 base pairs for the primers. The molecular identification using the 658-bp fragment of the (COI) gene was congruent with the morphological identification.

Keywords:

Morphological, molecular identification, cytochrome c oxidase gene subunit I

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Author Biographies

Godwin Kwakye-Nuako, Department of Biomedical Sciences, School of Allied Health Sciences, University of Cape Coast, Ghana

Seth Offei Addo, Noguchi Memorial Institute for Medical Research, Parasitology Department, University of Ghana, Legon

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