Comparing the Neuroprotective Effects of Aqueous and Methanolic Extracts of Vernonia Amygdalina on the Cerebellum of Adult Male Wistar Rats

Ugochukwu Samuel Aguwa; Somadina M Okeke; Chijioke M Okeke; Chinyere E Eze; Benedict Nzube Obinwa; Morris Izuogu; Felix Ogbo Ovie; Darlington Nnamdi Onyejike; Doris Kasarachi Ogbuokiri; Damian N Ezejindu

doi:10.21467/ias.9.1.145-159

Authors

Ugochukwu Samuel Aguwa Nnamdi Azikiwe University, Nnewi campus
Somadina M Okeke Nnamdi Azikiwe University, Nnewi campus
Chijioke M Okeke Nnamdi Azikiwe University, Nnewi campus
Chinyere E Eze Nnamdi Azikiwe University, Nnewi campus
Benedict Nzube Obinwa Nnamdi Azikiwe University, Nnewi campus
Morris Izuogu Nnamdi Azikiwe University, Nnewi campus
Felix Ogbo Ovie Nnamdi Azikiwe University, Nnewi campus
Darlington Nnamdi Onyejike Nnamdi Azikiwe University, Nnewi campus
Doris Kasarachi Ogbuokiri Nnamdi Azikiwe University, Nnewi campus
Damian N Ezejindu Nnamdi Azikiwe University, Nnewi campus

DOI:

https://doi.org/10.21467/ias.9.1.145-159

Abstract

The solvent of extraction determines the type and amount of phytochemicals extractible from a plant material. Reports have shown that numerous African herbs are rich is potent phytochemicals which have been used in folkloric and orthodox medicine in the prevention and treatment of ailments. This research is geared towards comparing the neuroprotective potency of the aqueous and methanolic extracts of Vernonia amygdalina on the cerebellum of mercury chloride intoxicated Wistar rats. Thirty adult male Wistar rats were divided into 6 groups of 5 rats each. Group A was the negative control and had food and water only. Group B was the positive control and was exposed to mercury chloride without treatment. Groups C1 and C2 received 200mg/kg bw of aqueous and methanolic extracts respectively while groups D1 and D2 received 400mg/kg bw of aqueous and methanolic extracts of Vernonia amygdalina respectively. Result of phytochemical analysis shows that water extracted more flavonoids while methanol extracted more phenols. The results of antioxidant studies show that the methanolic extract conferred more protection against oxidative stress than the aqueous extract. Histological results show that aqueous extract conferred more protection than the methanolic extract. We therefore conclude from our report that both the aqueous and methanolic extracts of Vernonia amygdalina confer neuroprotection on the cerebellum of Wistar rats.

Keywords:

Methanol, Aqueous, Neuroprotective, Cerebellum, Vernonia amygdalina, Antioxidant

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References

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