Anti-nutritional Composition, Heavy Metal Content and Mineral Bioavailability of Red Tree Vine (Leea guineensis G. Don) Fruits

Musbau Bayonle Olaniyii; Samsideen Olabiyi Rufai

doi:10.21467/ias.9.1.70-79

Authors

Musbau Bayonle Olaniyii Biomedicinal Research Centre, Forestry Research Institute of Nigeria
Samsideen Olabiyi Rufai Department of BioMedicinal Research Centre, Forestry Research Institute of Nigeria

DOI:

https://doi.org/10.21467/ias.9.1.70-79

Abstract

Leea guineensis G. Don is an evergreen shrub that has been reported to be important in Nigeria due to its medicinal and nutritional uses. However, there is paucity of documented information on the anti-nutritional composition, heavy metal content and mineral bioavailability of L. guineensis whole fruits. Hence, this study investigated the essential minerals, heavy metals and anti-nutritional compositions of L. guineensis whole fruits with a view to assess the quantity, bioavailability of the mineral elements and ultimately the safety of the fruits using standard analytical procedures. The results of phytochemical screening confirmed presence of secondary metabolites in varying quantities. The anti-nutrient contents (mg/100g) were phytates (29530); oxalates (510); saponins (10333); alkaloids (30533) and tannins (53.3). The results of mineral analysis revealed that L. guineensis fruits were rich in essential minerals (mg/100g) like, potassium (493); calcium (200); magnesium (103.3); phosphorus (9.9); zinc (8.5); manganese (5.5) and iron (5.0). The heavy metals concentrations were in the order lead>chromium>nickel>cobalt>cadmium. The values obtained were within the WHO permissible limits for heavy metals in medicinal plants. Meanwhile, the results of molar ratio phytates: Ca (8.97); oxalates: Ca (0.7978) and Ca.phytates:Zn (0.64) were below the suggested critical values indicating the bioavailability of calcium to be high. While phytates: Fe (501.06) and phytates: Zn (348.22) were higher than the critical values indicating poor bioavailability of Fe and Zn. The study therefore suggests that L. guineensis fruits are a good source of phyto-minerals which can be harnessed for nutritional purpose. Also, the anti-nutrient contents in L. guineensis whole fruits should be reduced to a safe level through processing methods (soaking, de-pulping or fermentation) in order to achieve its optimum nutritional or medicinal use.

Keywords:

Anti-nutrients, Bioavailability, Heavy metal, Mineral composition, Leea guineensis, wild fruits

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References

H.N. Ene-obong, H.O. Okadu and U.V. Asumugba. ‘’Nutrient and phytochemical composition of two varieties of monkey kola (Cola parchycarpa and Cola lepidota): an underutilised fruits’’. Journal Food Chemistry, 193, 154 – 159, 2016.

S. Yashaswini, R.V. Hedge and C.K. Venugopal. ‘’Health and Nutrition from ornamentals’’. International Research Journal of Pharmacy, 2(2), 375 – 382, 2011.

K. Asha, K.P. Asish, J.R. Kumar and P. Ashok. ‘’Antioxidant activities, metabolic profiling, proximate analysis, mineral nutrient composition of Salvadora persia fruit: Unravel potential functional food and a natural source of pharmaceuticals’’. Frontier Pharmacology, 8 (61), 1 – 14, 2017.

S.S. Anita, E.J. Akpan, P.A. Okon and I.U. Umoren. Proximate composition and phytochemical constituents of leaves of some Acalypha species. Pakistan Journal of Nutrition. Vol. 5, 166 – 168, 2006.

C.P. Sanchez-castillo and W.P. James. The mineral content of Mexican fruits and vegetables. Journal of Food Composition and Analysis, Vol. 11(4), 340 – 356, 1998.

C.U. Ogunka-Nnoka and H.D. Mepba, ‘’Proximate composition and anti-nutrient contents of some common spices in Nigeria’’ The Open Food Science Journal, vol. 2, pp. 62 – 67, 2008.

M.B. Olaniyi, I.O. Lawal and A.A. Olaniyi, ‘’Proximate, phytochemical screening and mineral analysis of Crescentia cujete L. leaves’’ Journal of Medicinal Plants for Economics Development, vol. 2, no. 1, pp. a28, 2018.

H.W. Lopez, F. Leenhardt, C. Coudray and C. Remesy, ‘’Minerals and phytic acid interactions - Is it a real problem for human nutrition‘’ Int. Journal of Food Sci. and Technol. vol. 37, pp. 727 – 739. 2002.

L. Hallberg, M. Brune and L. Rossander, ‘’Iron absorption in man: Ascorbic acid and dose-dependent inhibition by phytate’’. The American Journal of Clinical Nutrition, vol. 49, pp. 140 – 144. 1989.

S.W. Park, ‘’Bioavailability analysis of oxalate and mineral content in selected edible mushrooms’’ Journal of Nutritional Disorders and Therapy, vol. 4, pp. 1 – 6, 2013.

S. Muhammad, L.G. Hassan, S.M. Dangoggo, S.W. Hassan, K.J. Umar and R.U. Aliyu, ‘’Nutritional and Anti-nutritional Composition of Sclerocarya birrea Seed-kernel’’ Studia Universitatis “Vasile Goldiş”, Life Sciences Series, vol. 21, no. 4, pp. 693 – 699, 2011.

B.O. Ajiboye, A.O. Oso and O.S. Kobomoje, ‘’Chemical composition and nutritional evaluation of Leea guineensis seed’’ Int. J. food Sci. Nutr. and Diet, vol. 3 no. 2, pp. 24 – 32, 2014.

P.A. Neji, O.A Ushie, H.A. Neji, I.J. Opara and O.O. Ojong, ‘’Screening and Antimicrobial Activity of Extracts of Leea guineensis Stem Bark’’ International Journal of Modern Chemistry, vol. 9, no. 1, pp. 1 – 9, 2017.

P. Kathirvel and P. Kumudha, ‘’Chemical composition of Prosopis juliflora (SW.) D.C (mosquito bean)’’ Int. J. Applied Biology Pharmaceutical Technology, vol. 2, pp. 5 – 14, 2011.

H.F. Gemede, N. Ratta, G.D. Haki, A.Z. Woldegiorgis and F. Beyene, ‘’Nutritional quality and health benefits of okra (Abelmoschus esculentus L.): a review’’ Journal of Food Process Technology, vol. 6, pp. 485, 2015.

V.A. Adekunle, O.V. Ogerinde, ‘’Food potentials of some indigenous wild fruit in lowland rainforests ecosystem of south west Nigeria’’ Journal of Food Technology, vol. 2, pp.12 – 130, 2004.

J.B. Harborne, Introduction to ecological biochemistry, 4th edition, Academic Press, London, United Kingdom, 1993.

G.E. Trease, and W.C. Evans, A textbook of Pharmacognosy, 13th edn., Bacilluere Tinal Limited, London, 1989.

A. Sofowora, Medicinal Plants and Traditional Medicine in Africa Spectrum Books Limited, Ibadan Nigeria, pp.191 – 289, 1993.

C. Quettier-Deleu, B. Gressier, J. Vasseur, T. Dine, C. Brunet and M. Luyckx, ‘’Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour’’ Journal of Ethno-pharmacology, vol. 72, pp. 35 – 42. 2000.

J.B. Harborne, Phytochemical methods – A guide to modern techniques of plant analysis, 3rd editon, Springer Pvt. Limited, New Delhi, 2005.

B.O. Obadoni and P.O. Ochuko, ‘’Phytochemical studies and comparative efficacy of the crude extract of some homeostatic plants in Edo and Delta states of Nigeria’’ Global Journal of Pure Applied science, vol. 86 pp. 203 – 208, 2001.

M.L. Price, S.W. Van Scoyoc and L.G. Butler, ‘’A critical evaluation of the vanillin reactions as an assay for tannins in sorghum grain’’ Journal of Agric. Food Chemistry, vol. 26, pp. 1214 – 1218, 1978.

E. Lister and P. Wilson, Measurement of total phenolics and ABTS Assay for Antioxidant Activity, Crop Research Institute Lincoln, New Zealand, 2001.

Association of Official Analytical Chemists international (AOAC), Determination of Lead, Cadmium, and Minerals in Foods by Atomic Absorption Spectrophotometry Method (999.11/985.35), Association of Official Analytical Chemists, Gaithersburg, MD, USA, 2005.

E.L Wheeler and R.E. Ferrel, ‘’A method for phytic acid determination in wheat and wheat fractions’’ Cereal Chemistry, vol. 48(3), pp. 312 – 320, 1971.

A. Sofowora, Medicinal Plants and Traditional Medicine in Africa. 2nd editiond, Spectrum Books Limited., Ibadan, Nigeria, vol. 151153, pp. 209 – 214, 2006.

A.Z. Woldegiorgis, D. Abate, G.D. Haki and G.R. Ziegler (2015). ‘’Major, minor and toxic minerals and anti-nutrient composition in edible mushrooms collected from Ethiopia’’ Food process Technology, vol. 6, pp.134 – 142, 2015.

S.D. Oyeyemi, S. Arowosegbe and A.O. Adebiyi, ‘’Phytochemical and Proximate Evaluation of Myrianthus arboreus (P. Beau) and Spargono sporgonophora (L.) Leaves 10SR’’ Journal of Agric. and Vet. Sci. vol. 7, no.9, pp. 125, 2014.

O.L. Awotedu, P.O. Ogunbamowo, I.B. Emmanuel and I.O. Lawal (2019). ‘’Phytominerals and phytochemical studies of Azadirachta indica, Leea guineensis and Parkia biglobosa’’. International Annals Science, vol. 6, no.1, 28 – 34, 2018.

D.E. Okwu, ‘’Phytochemicals, Vitamins and Minerals contents of two Nigeria Medicinal Plants’’ International Journal of Molecular Medicinal Advanced Science, vol. 1, no. 4, pp. 140 – 147, 2005.

E.D. Fagbohun, O.U. Lawal and M.E. Ore, ‘’The Proximate, Mineral and Phytochemical Analysis of the leaves of Ocimum gratissimum L., Melanthera scandens A. and Leea guineensis L. and their Medicinal value’’ International Journal of Applied Biology and Pharmaceutical Technology, pp. 15 – 20, 2012.

W.H. Lewis and M.P. Elvis-Lewis, ‘’Medicinal Plants as sources of new therapeutics’’ Ann. M. Bot. Gard. vol. 82, pp. 16 – 24, 1995.

D.E. Okwu, ‘’Phytochemicals, Vitamins and Minerals contents of two Nigeria Medicinal Plants’’ International Journal of Molecular Medicinal Advanced Science, vol. 1, no. 4, pp. 140 – 147, 2001.

M.T. Olaleye, ‘’Cytotoxicity and Antibacterial Activity of Methanolic Extract of Hibiscus sabdariffa’’ Journal of Medicinal. Plants Research, vol. 1, pp. 9 – 13. 2007.

L. Taylor, Bitter melon, “Herbal properties and actions’’, in L. Taylor (ed.), The healing Power of rainforest herbs, Rain tree Nutrition Incorporation., New York, pp. 1 – 5, 2005.

H. Fekadu, F. Beyene and G. Desse, ‘’Effect of traditional processing methods on nutritional composition and anti-nutritional factors of anchote (Coccinea abyssinica (Lam.) Cogn) tubers grown in western Ethiopia’’ Journal of Food Process Technology, vol. 4, pp. 249, 2013.

M.O. Bello, O.S. Falade, S.R. Adewusi and N.O. Olawore, ‘’Studies on the chemical compositions and anti-nutrients of some lesser known Nigeria fruits’’ African Journal of Biotechnology, vol. 7, no. 21, pp. 3972 – 3979, 2008.

M.A. Achaglinkame, R.O. Aderibigbe, O. Hensel. B. Sturm and J.K. Korese, ‘’Nutritional characteristics of four Underutilized edible wild fruits of dietary interest in Ghana’’ Foods vol. 8, no. 3, pp. 104, 2019.

O. A. Matthew, I. Hashim, O.B. Titilayo, B.S. Rasaq, Z.M. Benjamin, J.F. Francis. ‘’Nutrient and Anti–Nutrient Composition of Shea (Vitellaria paradoxa C. F. Gaertn) Kernel and Pulp in the North East Nigeria’’. International Journal of Sciences, vol 7 (9):56 – 66, 2018.

E. Ekpa and D. Sani. Phytochemical and anti-nutritional studies on some commonly consumed fruits in Lokoja, Kogi state of Nigeria, General Medicine Open. Vol. 2(3): pp. 1 – 5, 2018.

L.U. Thompson, ‘’Potential health beneﬁts and problems associated with anti-nutrients in foods’’ Food Research International, vol. 26, pp. 131 – 149, 1993.

B.Q. Phillippy, M. Lin and B. Rasco, ‘’Analysis of phytate in raw and cooked potatoes’’ Journal of Food Composition Analysis, vol. 17, pp. 217 – 226, 2004.

E.O. Adeparusi, ‘’Effect of processing on the nutrients and anti-nutrients of Lima bean (Phaseolus lunatus L.) flour’’ Nahrung, vol. 4, pp. 94 – 96, 2001.

H.F. Gemede and N. Ratta, ‘’Anti-nutritional factors in plants foods: potential health benefits and adverse effects’’ International Journal of Food Science, vol. 3, pp. 284 – 289, 2014.

I. Muhammed, S. Muh, S. Olorunju, J. Bale, U. Abdullahi, R. Lawal, ‘’Response of nutrients and anti-nutritional constituents in the seeds of Cassia tora (Linn.) to treatments’’ J. Agric. Environ., vol. 3, pp. 225–234, 2002.

C.J. Rosen, ‘’Sugar and Bone: A Not-So Sweet Story’’ Journal of Bone and Mineral Research, vol. 23, no. 12, pp. 1881 – 1883, 2009.

I.O. Olayiwola, V.O. Akinfenwa, C.O. Oguntona, S.A. Sanni, O.O. Onabanjo and W. A. Afolabi, ‘’Phytonutrient, antioxidant and mineral composition of some wild fruits in south West Nigeria’’ Nigerian Food Journal, www.nifst.org NIFOJ, vol. 31, no. 2, pp. 33 – 40, 2013.

A. Kermanshah, P. Ziarati, J. Asgarpanah and M. Qomi, ‘’Food values of two endemic wild Almond species from Iran’’ Int. J. Plant Animal and Environmental Sciences, vol.4, pp. 380 – 388, 2003.

A.M. Mlitan, M.S Sasi and A.M. Alkherraz, ‘’Proximate and minor mineral content in some selected basil leaves of Ocimum gratissimum L. in Libya’’ Int. J. Chem. Eng. Appl., vol. 5, 8 – 17, 2014.

R. Ullah, J.A. Khader, I. Hussain, N.M. AbdElsalam, M. Talha and N. Khan, ‘‘Investigation of macro micro-nutrients in selected medicinal plants’’ African Journal of Pharmacy and Pharmacology, vol. 6, no. 25, pp. 1827 – 1832, 2012.

D.L. Longo and C. Camaschella, ‘’Iron Deficiency Anaemia’’ New England Journal of Medicine, vol. 373, no. 5, pp. 484 – 486, 2015.

C. Breymann, ‘’Iron Deficiency Anaemia in Pregnancy – a review’’ Semin Hematol, vol. 52, no. 4, pp. 339 – 347, 2015.

J.C. Okaka and A.N. Okaka, Food Composition, Spoilage and Shelf life extension, ocjarc, Academic Publishers, Enugu, Nigeria, pp. 54 – 56, 2001.

A. Prasad, (Ed.) Essential and toxic element: Trace elements in human health and disease. Amsterdam, Elsevier, 2013.

B.O. Lonneral, ‘’Dietary factors influencing Zinc absorption’’ Journal of Nutrition, vol.130, no. 5, pp. 13785 – 13835, 2000.

R.F. Hurrell, M.B. Reddy, M.A. Juillerat and J.D. Cook, ‘’Degradation of phytic acid in cereal porridges improves iron absorption by human subjects’’. American Journal Clinical Nutrition, vol. 77, pp. 1213 – 1219, 2003.

M.R. Bhandari and J. Kawabata. ‘’Assessment of anti-nutritional factors and bioavailability of calcium and zinc in wild yam (Dioscorea spp.) tubers of Nepal’’. Food Chemistry, vol. 85, pp. 281 – 287, 2004.

E. Morris and R. Ellis, ‘’Usefulness of the dietary phytic acid/zinc molar ratio as an index of zinc bioavailability to rats and humans’’ Biological Trace Element Research, vol. 19, pp. 107 – 117, 1989.

C. Frontela, M.L. Scarino, S. Ferruzza, G. Ros and Martinez, ‘’Effect of dephytinization on bioavailability of iron, calcium and zinc from infant cereals assessed in the Caco-2 cell model’’ World Journal of Gastroenterology, vol. 28, pp. 1977 – 1984, 2009.

G. Obah and T.V. Amusa, ‘’Nutritive value and antioxidant properties of cereal gruels produced from fermented maize and sorghum’’ Food Biotechnology, vol. 23, pp. 17 – 31, 2009

F.O. Adetuyi, A.U. Osagie and A.T. Adekunle, ‘’Nutrient, anti-nutrient, mineral and bioavailability of Okra (Abelmoschus esculentus (L.) Moench)’’ American Journal of Food Nutrition, vol. 1, pp. 49 – 54, 2011.