Induction of Somatic Embryogenesis from Cocoa Farmer Field Collection of ICT - Peru

Mar AsunciÃ³n Garate-Navarro; Enrique ArÃ©valo-Gardini

doi:10.21467/ias.2.1.6-11

Authors

Mar AsunciÃ³n Garate-Navarro Instituto de Cultivos Tropicales
Enrique ArÃ©valo-Gardini Instituto de Cultivos Tropicales - ICT https://orcid.org/0000-0002-1725-6788

DOI:

https://doi.org/10.21467/ias.2.1.6-11

Abstract

To induce the formation of embryogenic structures from staminodes, in 10 cocoa genotypes (Theobroma cacao L.) from the ICT Collection, the influence of the combination of two growth hormones (2,4 Dichlorophenoxyacetic 2,4 -D and Thidiazuron TDZ) was studied. Floral explants (staminodes), were induced to form calli in a culture medium supplemented with modified DKW mineral salts. At 12 days of implantation in the culture medium, the explants responded to the callus induction with a bulge at the base, covering the explants totally with callus at 40 days of culture. Embryogenic expression was shown at 52 days of culture. The genotypes ICT-2161 and ICT-2142 shows the highest percentage of somatic embryogenesis. Four types of callus, fine crystalline, thick crystalline, semi-compact and compact were observed. In addition, a clear organogenic response was observed when roots were formed from the callus generated.

Keywords:

Tissue culture, tropical crops, in vitro propagation, micro propagation

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Author Biography

Enrique ArÃ©valo-Gardini, Instituto de Cultivos Tropicales - ICT

Coordinador General, Instituto de Cultivos Tropicales (ICT), Av Ahuashiyacu S/N, Cuadra 16, Sector Laguna Venecia, Banda del Shilcayo
Referencia( Esquina Esquina Mz. L, Urb. Santa Barbara y Ahuashiaco), Tarapoto, San MartÃn, PerÃº, Casilla Postal SERPOST NÂº 201

References

R.J. Hunter, The status of cocoa (Theobroma cacao, Sterculiaceae) in the western hemisphere. Econ Bot 44:425–439. 1990

A.I. Urrea-Trujillo, L. Atehortúa-Garcés, A.M. Gallego-Rúa, Regeneration through somatic embryogenesis of an elite colombian Theobroma cacao L. variety. Rev. Colomb. Biotecnol. 13 (2): 39-50. 2011.

S.N. Maximova, L. Alemano, A. Young, N. Ferriere, A. Traore, M.J. Guiltinan, Efficiency, genotypic variability, and celular origin of primary and scecondary somatic embryogenesis of Theobroma cacao L. In Vitro Cellular and Developmental Biology – Plant, v.38, p. 252-259. 2002

A. Chávez, and J. Mansilla, Manual del cultivo de cacao. Perú: Ministerio de Agricultura, Programa para el Desarrollo de la Amazonia. pp. 100. 2004

J. Silva, S. Montes, L. Acosta, E. Arias and A. García, Embriogénesis Somática una alternativa para la Propagación, Mejoramiento y Conservación de Germoplasma en Cacao. Cuba 9-12 pp. 2006

M.J. Guiltinan, S.N. Maximova, College of Agricultural Sciences. Integrated System for Vegetative Propagation of Cacao. Protocol Book. Pennsylvania State University. Version 2.1 4-24pp. 2010

D. Driver and D. Kuniyuki, In vitro propagation of Paradox walnut rootstock. HortScience, 19: 507-509. 1984

G. Lloyd and B. Mc Cown, Woody Plant Medium: A mineral nutrient formulation for microculture of woody plant species. HortScience, 16: 453. 1981

J.A. Di Rienzo, F. Casanoves, M.G. Balzarini, L. Gonzalez, M. Tablada and C.W. Robledo, InfoStat versión 2014. Grupo InfoStat, FCA, Universidad Nacional de Córdoba, Argentina. URL http://www.infostat.com.ar. 2014

B. Quimbita, Efecto de la concentración de 2,4-D y el tipo de explante en la formación de callos embriogénicos en el cultivo in vitro de cacao (Theobroma cacao L.). Trabajo de diploma. Universidad de Granma. Facultad de Ciencias Agrícolas -Centro de Estudios de Biotecnología Vegetal. Bayamo, CU. pp. 33-43. 2011

C. Chanatásig, “Inducción de la embriogénesis somática en clones superiores de cacao (Theobroma cacao L.), con resistencia a enfermedades fungosas”. Thesis. CATIE, Turrialba, Costa Rica. 2004

G.F. Santana, R.Velásquez and J. Mata, Carbon source effect on cacao organogenesis and somatic embryogenesis. Citogenetic analysis. Agronomía Trop. 60 (2). 12p 2010

K. Díaz, C. Valiente, M. Martínez, M. Castillo and E. Sanfuentes, Root-promoting rhizobacteria in Eucalyptus globulus cuttings. World Journal of Microbiology and Biotechnology, v. 25, n. 5, p. 867-873, 2009.

L. Monsalve, C. García and A. Sigarroa, Obtención de embriones somáticos primarios de Theobroma cacao en clones de interés regional para el departamento Norte de Santander, Colombia. Revista Respuestas, 1: 21-28. 2005

C. Tan and D. Furtek, Development of an in vitro regeneration system for Theobroma cacao from mature tissues. Plant Science, 164: 407-412. 2003

Z. Li, A. Traore, S.N. Maximova, M.J. Guiltinan, Somatic embryogenesis and plant regeneration from Floral explants of cacao (Theobroma cacao L.) using thidiazuron. In Vitro Cell Dev Biol Plant 34:293–299pp. 1998

P.V. Ammirato, Embryogenesis. In: D.A. Evans, W.R. Sharp, P.V. Ammirato and Y. Yamada, Handbook of plant cell culture, V. I. Kluwer Academic Publisher, Dordreh, p. 81-181. 1993

V. Jiménez, Involvement of plant hormones and plant growth regulators on in vitro somatic embryogenesis. Plant Growth Regulation 47:91–110. 2005