Preparation and Evaluation the Efficacy of Combined Mixture of Finger Root and Robusta Coffee Extracts on Antioxidative Activity


  • Chotirod Kitsomchip Faculty of Pharmaceutical Sciences, Huachiew Chalermprakiet University, Samutpakan, Thailand
  • Ratana Indranupakorn Faculty of Pharmaceutical Sciences, Huachiew Chalermprakiet University, Samutpakan, Thailand



Finger Root (FR) and Robusta Coffee (RC) are natural substances known to be beneficial for human health and used as a traditional medicine in various parts of the world. The efficacy of two commonly used extraction techniques [Ultrasound-Assisted Extraction (UAE) and maceration with stirring (MR)] and four different extraction solvents (50% ethanol, 80% ethanol, 100% ethanol and acetone) on antioxidant activity and the Total Phenolic Content (TPC) in both herbs were investigated. The antioxidant potentials of the samples were determined by reducing capacity with Folin-Ciocalteu reagent, while the radical scavenging activity using 1,1-Diphenyl2-Picryl Hydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothia zoline-6-sulfonic acid) (ABTS) assays. FR and RC extracts from UAE and using 80% and 50% ethanol as an extraction solvent, respectively, showed the highest antioxidant capacity compared to the other extraction processes. The combined mixtures of FR and RC (CFR) in the weight ratios of 1:1 was prepared and evaluated the antioxidant activity. The CFR extract showed a significant increase of TPC and resulted in its strong radical scavenging activity, quite the same level in comparison to the model antioxidant substance, ascorbic acid, compared to when they were assayed independently. Stability results indicated that the TPC of CFR extract was slightly decreased, by around 8.7% and the IC50 values of CFR extract showed slightly increased from 7.13 to 9.06 µg/mL in DPPH assay and from 725.72 to 858.65 µg/mL ABTS assay after storage at room temperature over 1 month. Findings suggested that the extraction methods and different solvent polarity significantly affect polyphenol recovery. The combined mixture of FR and RC extracts, which is quite stable and enhances radical scavenging activity, is interesting to increase economic value and utilization in cosmetic industry.


Fingerroot, Robusta coffee, Antioxidant Activity, Total Phenolic Content


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U. Shabbir, A. Tyagi, F. Elahi, S. Okomo Aloo and D.H. Oh, “The Potential Role of Polyphenols in Oxidative Stress and Inflammation Induced by Gut Microbiota in Alzheimer’s Disease,” Antioxidants, vol. 10, no. 9, pp. 1370-1388, 2021.

S. Chandra and D. Lo, “Functional properties of Boesenbergia pandurata and Murraya paniculata: A review,” IOP Conf. Ser.: Earth Environ. Sci., vol. 794 012151, pp. 1-10, 2021.

C. Mamak, J. Duangdamrong, T. Phayakkhawisai, B. Trakoolsilp, K. Jamparngernthaweesri, P. Sukcharoen, P. Seeloopmok, P. Akarasereenont and N. Lumlerdkij, “Evaluation of Evidence Related to Medical Uses and Health Claims of Fingerroot,” Siriraj Medical Bulletin, vol. 14, no. 2, pp. 61-72, 2021.

P.F. Pinheiro, C.A. Pinheiro, V.M.Osorio and L.L.Pereira, “Chemical Constituents of Coffee,” In book: Quality Determinants In Coffee Production. pp. 209-254, 2021. DOI:10.1007/978-3-030-54437-9_5.

L. Barrea, G. Pugliese, E. Frias-Toral, M. El.Ghoch, B. Castellucci, S.P. Chapela, M. Carignano, D. Laudisio, S. Savastano, A. Colao and G. Muscogiuri, “Coffee consumption, health benefits and side effects: a narrative review and update for dietitians and nutritionists,” Critical Reviews in Food Science and Nutrition, Aug 28, pp.1-24. 2021. DOI: 10.1080/10408398.2021.1963207

S.A. Saquib, N.A. AlQahtani, I. Ahmad, S. Arora, S.M. Asif, M.A. Javali and N. Nisar, “Synergistic antibacterial activity of herbal extracts with antibiotics on bacteria responsible for periodontitis,” J Infect Dev Ctries, Nov 30; vol. 15, no.11, pp.1685-1693, 2021. DOI: 10.3855/jidc.14904. PMID: 34898497.

N. Siddiqui, A. Rauf, A. Latif and Z. Mahmood, “Spectrophotometric determination of the total phenolic content, spectral and fluorescence study of the herbal Unani drug Gul-e-Zoofa (Nepeta bracteata Benth),” J. Taibah Univ. Sci. vol. 12, pp. 360-363, 2017.

R. Ahmad, A.M. Ali, D.A. Israf, N.H. Ismail, K. Shaari and N.H. Lajis, “Antioxidant, radical-scavenging, anti-inflammatory, cytotoxic and antibacterial activities of methanolic extracts of some Hedyotis species,” Life Sci., vol. 76, pp.1953-1964, 2005.

R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M.Yang and C. Rice-Evans, “Antioxidant activity applying an improved ABTS radical cation decolorization assay,” Free Radical Biology and Medicine, vol. 26 (9–10), p. 1231-1237, 1999.

K. Sooksing and W. Jirapakkul, “Effect of drying on flavonoid content and antioxidant capacity in fingerroots (Boesenbergia pandurata),” In Proceedings of the 49th Kasetsart University Annual Conference, vol. 7, pp. 641-648, 2011.

N. Saewan, A. Jimtaisong and W. Vichit, “Optimization of phenolic extraction from coffee by - product using response surface methodology and their antioxidant activities,” FABJ, vol. 8, pp. 14-26, 2020.

A.I. Hussain, S.A.S. Chatha, S. Noor, M.U. Arshad, Z.A. Khan, H.A. Rathore and M.Z.A. Sattar, “Effect of Extraction Techniques and Solvent Systems on the Extraction of Antioxidant Components from Peanut (Arachis hypogaea L.) Hulls,” Food Anal. Methods, 5(4), pp.890-896, 2012,

G. J. Molole, A. Gure and N. Abdissa, “Determination of total phenolic content and antioxidant activity of Commiphora mollis (Oliv.) Engl. Resin,” Molole et al. BMC Chemistry, pp. 1-11, 2022.

B. Ulewicz-Magulska and M. Wesolowski, “Total Phenolic Contents and Antioxidant Potential of Herbs Used for Medical and Culinary Purposes,” Plant Foods for Human Nutrition, pp. 61–67, 2019.






Graduate Research Articles

How to Cite

C. Kitsomchip and R. Indranupakorn, “Preparation and Evaluation the Efficacy of Combined Mixture of Finger Root and Robusta Coffee Extracts on Antioxidative Activity”, Adv. J. Grad. Res., vol. 13, no. 1, pp. 1–7, Dec. 2022.