Advanced Journal of Graduate Research https://journals.aijr.org/index.php/ajgr <p align="justify"><a title="Click for Journal homepage" href="https://doi.org/10.21467/ajgr" target="_blank" rel="noopener"><img style="float: right; padding-left: 15px; padding-right: 5px;" src="https://journals.aijr.org/public/site/images/aabahishti/AJGR_Cover_Page.jpg" alt="AJGR" /></a>Advanced Journal of Graduate Research is a multidisciplinary, international journal featuring the work of graduate students and young researchers. This journal seeks to disseminate the work of emerging students who focus on scientific research, regardless of their academic discipline. <em>Adv. J. Grad. Res.</em> publishes research carried out by graduate students and young researchers (Bachelor's degree students and Master's degree students) that sound scientifically and technically valid. This journal will serve as a global platform to broadcast new research initiatives being carried out by today's brightest youths as part of their graduate project.<br />Advanced Journal of Graduate Research is published by AIJR publisher (India) and registered with CrossRef with doi: 10.21467/ajgr and ISSN of this journal is 2456-7108 [online].</p> AIJR Publisher en-US Advanced Journal of Graduate Research 2456-7108 <div id="copyrightNotice">Author(s) retains full copyright of their article and grants non-exclusive publishing right to this journal and its publisher <a title="About AIJR Publisher" href="https://aijr.org/about/about-aijr/" target="_blank" rel="noopener">AIJR</a> (India). Author(s) can archive pre-print, post-print, and published version/PDF to any open access, institutional repository, social media, or personal website provided that Published source must be acknowledged with citation and link to publisher version.<br />Click <a title="Copyright Policy" href="https://aijr.org/about/policies/copyright/" target="_blank" rel="noopener">here</a> for more information on Copyright policy<br />Click <a title="Licensing Policy" href="https://journals.aijr.org/index.php/ajgr/about#licensing" target="_blank" rel="noopener">here</a> for more information on Licensing policy</div> An In-Gel Fluorescence Technique to Study Autophagy in Human Embryonic Kidney 293 Cells https://journals.aijr.org/index.php/ajgr/article/view/10121 <p class="Abstract"><span lang="EN-US">Autophagy is a recycling pathway used by cells to maintain homeostasis during times of stress. Common methods for the analysis of autophagy include western blotting and fluorescent imaging, but these methods are time consuming, expensive, and complex, making them inherently difficult for research laboratories at primarily undergraduate research institutions. We propose an in-gel fluorescence method for the analysis of autophagy in cells transfected with a dual-reporter plasmid. We verify that this method allows users to detect autophagic stimulation and is a cost effective, less complex method that sidesteps the challenges inherent with existing methods.</span></p> Melissa Rogers Riley Hatton Robert Youker Heather Coan Copyright (c) 2026 Melissa Rogers, Riley Hatton, Robert Youker, Heather Coan http://creativecommons.org/licenses/by-nc/4.0 2026-04-14 2026-04-14 17 1 1 10 10.21467/ajgr.17.1.1-10