Phytochemical Screening of Selected Botanicals and their Effectiveness Against Maize Weevil (Sitophilus Zeamais Motsch.) at Paklihawa, Rupandehi, Nepal

Susmita Gautam; Subodh Khanal; Dipak Khanal; Saroj Raj Mishra; Sunil Ghimire

doi:10.21467/ajgr.11.1.34-44

Authors

Susmita Gautam Department of Agri-botany and Ecology, Institute of Agriculture and Animal Science, Tribhuwan University
Subodh Khanal Department of Agri-botany and Ecology, Institute of Agriculture and Animal Science, Tribhuwan University https://orcid.org/0000-0002-7326-3560
Dipak Khanal Department of Entomology, Institute of Agriculture and Animal Science, Tribhuwan University
Saroj Raj Mishra Department of Agri-botany and Ecology, Institute of Agriculture and Animal Science, Tribhuwan University
Sunil Ghimire Department of Soil Science and Agri-engineering, Agriculture and Forestry University https://orcid.org/0000-0003-2741-8389

DOI:

https://doi.org/10.21467/ajgr.11.1.34-44

Abstract

Laboratory experiment was done to perform the phytochemical screening of six locally available botanicals (Azadirachta indica Juss, Artemisia dubia Wall, Lantana camara L, Parthenium hysterophorus L, Justicia adhatoda Linn. and Moringa oleifera L.) and their effectiveness to control the maize weevil (Sitophilus zeamais Motsch.) at Agriecology lab of Institute of Agriculture and Animal Science, Paklihawa Campus, Rupandehi, Nepal. Seven treatments (leaf extract of A. indica, A. dubia, L. camara, P. hysterophorus. J. adhatoda and M. oleifera and distilled water as control) applied at 3 different doses (0.5, 1 & 2g/50g seeds) were arranged in CRD design with 3 replications. Result of mortality test of maize weevil revealed that, at highest concentration of L. camara powder (T17- 2g/50 gm seeds) mortality of maize weevil was observed up to 85% at 45 DAT. Whereas number of damaged grain was lowest in T17 (L. camara powder- 2g/50 gm seeds). The effectiveness of botanicals was found to increase with increasing the concentration. Besides that, Justicia adhatoda (2g/50g seeds) and Artemisia dubia (1g/50g seeds) were also found as good candidates to use for the control of Sitophilus zeamais. Qualitative tests done for the presence of various phytochemicals showed that Azadirachta indica Juss and Artemisia dubia Wall. were found to contain most of the tested metabolites among all botanicals. Most of those phytochemicals were extracted by the aqueous solution rather than other 3 menstrums used (acetone, methanol and petroleum ether). Similarly, quantitative test revealed that percentage of alkaloid was found 18.01%, 17.78%, 17.75%, 11.58%, 8.20%, 6.78% and flavonoid15.32%, 13.63%, 12.86%, 11.36%, 7. 51% 2.85% respectively in L. camara L. P. hysterophorus L., J. adhatoda Linn., M. oleifera L., A. indica Juss. and A. dubia Wall. Leaf extract of L. camera found to contain highest percentage of alkaloid (18.01%) as well as flavonoid (15.32%). So, it was concluded that locally available botanicals extract reach in alkaloid and flavonoid may have insecticidal property and could be use as grain protectant against the maize weevil in cost effective and eco-friendly manner.

However, application at proper dose is necessary to maintain the efficacy at higher level.

Keywords:

Phytochemicals, Botanicals, Mortality, Maize weevil

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References

MoALD, Statistical Information on Nepalese Agriculture 2018/19, Ministry of Agriculture and Livestock Development (MoALD), Kathmandu, Nepal, Aug 2020.

T. F. Erenso and D. H. Berhe. "Effect of neem leaf and seed powders against adult maize weevil (Sitophilus zeamaisMotschulsky) mortality.", Int. J. Agric. Res, Vol. 11, pp. 90-94, 2016.

E. N. Nukenine. "Stored product protection in Africa: Past, present and future.", Julius-Kühn-Archiv, Issue 425, pp. 26, Sept 2010. DOI: 10.5073/jka.2010.425.177

L. C. Nwosu. "Impact of age on the biological activities of Sitophilus zeamais (Coleoptera: Curculionidae) adults on stored maize: implications for food security and pest management.", Journal of economic entomology, Vol. 111 Issue 5, pp. 2454-2460, Oct 2018. https://doi.org/10.1093/jee/toy187

D. Khanal, S. B. Neupane, A. Bhattarai, S. Khatri-Chhetri, N. Nakarmi, S. Sapkota and V. Sharma. "Evaluation of Botanical Powders for the Management of Rice Weevil (Sitophilus oryzae L. Coleoptera: Curculionidae) in Rupandehi, Nepal.", Advances in Agriculture, Jan 2021. https://doi.org/10.1155/2021/8878525

D. Webb, “Phytochemicals’ role in good health.”, Today’s Dietitian, Vol. 15 Issue 9, p.70, 2013.

M. A. Motalab, “Selected medicinal plants of Chittagong hill tracts. IUCN Bangladesh.”, 2011.

S. Rajni and S. Priyam, “Study of insecticidal activity of acetone crude leaf extract of Adhatodavasica against Callosobruchus maculates.”, World Journal of Pharmacy and Pharmaceutical Sciences, Vol. 3, Issue9, pp. 1573–1577, 2014.

A. Trivedi, N. Nayak, and J. Kumar. "Recent advances and review on use of botanicals from medicinal and aromatic plants in stored grain pest management.", Journal of Entomology and Zoology Studies, Vol. 6 Issue 3, pp. 295-300, Apr 2018.

A. Prakash and J. Roa, Botanical Pesticides in Agriculture Lewis Publishers, 1997.

V. D. Brito, F. Achimón, R. P. Pizzolitto, A. R. Sánchez, E. A. G. Torres, J. A. Zygadlo and M. P. Zunino. "An alternative to reduce the use of the synthetic insecticide against the maize weevil Sitophilus zeamais through the synergistic action of Pimentaracemosa and Citrus sinensis essential oils with chlorpyrifos.", Journal of Pest Science, Vol. 94, Issue 2, pp. 409-421, March 2021. https://doi.org/10.1007/s10340-020-01264-0

S. Karunakaran and V. Arulnandhy. "Insecticidal activity of selected botanicals on maize weevil, Sitophilus zeamais L., in stored maize grains.", AGRIEAST: J. Agric. Sci, Vol. 12, Issue1, pp. 1-6, June 2018. DOI: http://doi.org/10.4038/agrieast.v12i1.46

K. D. Ileke and M. O. Oni. "Toxicity of some plant powders to maize weevil, Sitophilus zeamais (motschulsky)[Coleoptera: Curculiondae] on stored wheat grains (Triticum aestivum).", African Journal of Agricultural Research, Vol. 6, Issue13, pp. 3043-3048, July 2011. https://doi.org/10.5897/AJAR.9000745

G. Visweswari, R. Christopher, and W. Rajendra, “Phytochemical screening of active secondary metabolites present in Withaniasomnifera root: role in traditional medicine.”, International journal of pharmaceutical sciences and research, Vol. 4, Issue 7, p. 2770, June 2013.

J. B. Harborne, “Phenolic compounds.”, In Phytochemical methods, pp. 33-88, 1973. doi: https://doi.org/10.1007/978-94-009-5921-7_2.

K. C. Ugoeze, P. C. Aja, N. Nwachukwu, B. C. Chinko& J. N. Egwurugwu. "Assessment of the phytoconstituents and optimal applicable concentration of aqueous extract of Azadirachta indica leaves for wound healing in male Wistar rats.", Thai Journal of Pharmaceutical Sciences (TJPS), Vol. 45, Issue 1, Jan 2021.

K. C. Ugoeze, K. E. Oluigbo, and B. C. Chinko. "Phytomedicinal and Nutraceutical Benefits of the GC-FID Quantified Phytocomponents of the Aqueous Extract of Azadirachta indica leaves.", Journal of Pharmacy and Pharmacology Research, Vol. 4, Issue 4, pp. 149-163, Dec 2020. DOI: 10.26502/fjppr.039

G. Gebremedhin, T. Tesfay, K. K. Chaithanya and K. Kamalakararao. "Phytochemical screening and in vitro anti-dandruff activities of bark extracts of neem (Azadirachta indica).", Drug. Invent. Today, Vol. 13, pp. 707-713, May 2020.

A. Uwague, "Comparative Potential Qualitative and Quantitative Phytochemical Evaluation of Neem and Moringa Oleifera Leaf Plants in Ozoro.", pp. 120-340. Nov. – Dec. 2019. doi: https://doi.org/10.32628/IJSRST196652

P. K. Ashok and K. Upadhaya, “Preliminary phytochemical screening and physico-chemical parameters of aerial parts of Artemisia vulgaris.”, International Journal of Research in Ayurveda and Pharmacy (IJRAP), vol. 1, Issue 1, pp. 206-211, 2010.

R. A. Sardhara and S. Gopal, “Qualitative phytochemical screening of different solvent extracts of tinosporacordifolia stem and lantana camara flower. Int. Res. J. Pharm.”, Applied Science, vol. 3, Issue 5, pp. 210–213, Oct 2013.

[M. A. Etuh, L. T. Ohemu, and D. D. Pam. "Lantana camara ethanolic leaves extracts exhibit anti-aging properties in Drosophila melanogaster: survival-rate and life span studies.", Toxicology Research, Vol. 10, Issue 1, pp 79-83, Jan 2021. doi: 10.1093/toxres/tfaa098

H. Gul and M. A. Sajad. "An evaluation of phytochemical screening of Lantana camara Linn. (An invasive plant species of Pakistan).", Pure and Applied Biology (PAB), Vol. 9, pp.1856-1863, Aug 2020. http://dx.doi.org/10.19045/bspab.2020.90198

E. Bhuvaneswari and R. S. Giri. "Physicochemical and phytochemical screening in Lantana camara leaves.", Journal of Pharmacognosy and Phytochemistry, Vol. 7, Issue 6, pp. 1962-1966, March 2018.

P. Pradhan and Y. S. Sarangdevot. "Estimation of total Phenols and flavonoids content in parthenium hysterophorus aerial parts.", Plant Archives, Vol. 20, Issue 1, pp. 988-990, 2020.

B. A. Ayinde, D. N. Onwukaeme, and E.K. Omogbai, “Isolation and characterization of two phenolic compounds from the stem bark of Musangacecropioides R. Brown (Moraceae),” Acta Pol. Pharm, Vol. 64, Issue2, pp. 183-5¸ March 2007. doi: 10.11648/j.ijnfs.20140306.23

M. B. Tijjani, I.A. Bello, A.B. Aliyu, T. Olurishe, S.M. Maidawa, J.D. Habila, and E.O. Balogun, “Phytochemical and antibacterial studies of root extract of Cochlospermumtinctorium A. Rich. (Cochlospermaceae),” Research Journal of Medicinal Plant, Vol. 3, Issue 1, pp. 16-22, 2009. doi: http://dx.doi.org/10.3923/rjmp.2009.16.22

M. L. Parugrug and A.C. Roxas, “Insecticidal action of five plants against maize weevil, Sitophilus zeamais Motsch,” (Coleoptera: Curculionidae). Current applied science and technology, Vol. 8, Issue 1, pp. 24–38, March 2018.

J. O. Ogendo, S. R. Belmain, A. L. Deng, and D. J. Walker, “Comparison of Toxic and Repellent Effects of Lantana camara L. with TePhrosiavogelii Hook and A Synthetic Pesticide Against Sitophiluszeamais Motschulsky (Coleoptera: Gurculionidae) in Stored Maize Grain.”, International Journal of tropical insect science, Vol. 23, Issue 2, pp. 127-135, June 2003. doi: https://doi.org/10.1017/S1742758400020348

M. A. Mahmoud and O. A. A. Zedan, “Essaying some Plant Powders against Maize Weevil, Sitophiluszeamais (Motsch.), for Protecting Maize Grains from Damage.”, Journal of Plant Protection and Pathology, Vol. 9, Issue 11, pp. 729-734, Nov 2018. doi: 10.21608/jppp.2018.44041.

A. A. Ayalew, "Insecticidal activity of Lantana camara extract oil on controlling maize grain weevils.", Toxicology Research and Application, 4, 2397847320906491. 2020. https://doi.org/10.1177/2397847320906491