Exploring Extracellular Vesicles Nanocapsules in Hydrogel Delivery for Canine Atopic Dermatitis

Thu, 05 Dec 2024 00:00:00 +0000

This study aims to evaluate the anti-inflammatory potential of nanoencapsulated extracellular vesicles (EV) derived from canine mesenchymal stem cells (MSCs) in treating atopic dermatitis. It seeks to determine the efficacy of these EV in modulating cytokine responses, specifically targeting IL-2, IL-4, IL-5, IL-6, IL-10, IL13, and IL-31, which are crucial in the pathophysiology of cutaneous inflammatory disorders. Materials and Methods: MSCs were cultured from adipose tissue and induced to produce EVs. They were then isolated through ultracentrifugation, characterized for particle size and morphology, and encapsulated within calcium alginate nanogels. The anti-inflammatory activity was assessed in vitro using human keratinocyte cultures primed with IFN-gamma and treated with both free and nanoencapsulated EV. The expression of inflammatory cytokines was analyzed through RTq-PCR and protein ELISA Multiplex (Luminex®). Results: The study found that both free and nanoencapsulated EV significantly reduced the expression of IL-2, IL-4, IL-5, IL6, IL-10, IL-13, and IL-31 in IFN-gamma primed keratinocytes. Nanoencapsulation with alginate notably enhanced the delivery efficiency, targeted release, and stability of the EV, leading to a more pronounced reduction in cytokine expression compared to free EV. Conclusion: Nanoencapsulated EV derived from canine MSCs demonstrate significant anti-inflammatory potential, offering a promising therapeutic strategy for atopic dermatitis and other inflammatory conditions. This approach not only preserves the bioactivity of EV but also improves their therapeutic applicability through enhanced delivery and stability. The findings underscore the potential of nanoencapsulation technology in advancing novel therapeutic strategies across human and veterinary medicine, highlighting the importance of cross-disciplinary research in developing effective healthcare solutions.

Advanced Nano Research

Exploring Extracellular Vesicles Nanocapsules in Hydrogel Delivery for Canine Atopic Dermatitis