https://journals.aijr.org/index.php/anr <p align="justify"><a title="Click for Journal homepage" href="https://doi.org/10.21467/anr" target="_blank" rel="noopener"><img style="float: right; padding-left: 15px; padding-right: 5px;" src="/public/site/images/aabahishti/ANR_Cover_Page.jpg" alt="ANR"></a> Advanced Nano Research (ANR) is a peer-reviewed, international and interdisciplinary open access research journal published by AIJR publisher (India). <em>Adv. Nan. Res.</em> focuses on all aspects of nanoscience and nanotechnology. This Journal will cover all interesting areas of nano research from basic aspects of nanoscience and nanoscale materials to practical applications of such materials.<br>Advanced Nano Research is registered with CrossRef with doi: 10.21467/anr having&nbsp;ISSN:&nbsp;2581-5164 [online].</p> AIJR Publisher en-US Advanced Nano Research 2581-5164 <div id="copyrightNotice"> <p>The author(s) retains full copyright of their article and grants non-exclusive publishing right to Advanced Nano Research and its publisher <a title="AIJR Publisher homepage" href="https://aijr.org/about/about-aijr/" target="_blank" rel="noopener">AIJR</a> (India). Author(s) can archive pre-print, post-print, and published version/PDF to any open access, institutional repository, social media, or personal website provided that Published source must be acknowledged with citation and link to publisher version.<br />Click <a title="Copyright Policy" href="https://aijr.org/about/policies/copyright/" target="_blank" rel="noopener">here</a> for more information on Copyright policy<br />Click <a title="Licensing Policy" href="https://journals.aijr.org/index.php/anr/about#licensing" target="_blank" rel="noopener">here</a> for more information on Licensing policy</p> </div> https://journals.aijr.org/index.php/anr/article/view/9099 <p>This study aims to evaluate the anti-inflammatory potential of nanoencapsulated extracellular vesicles (EV) derived from canine mesenchymal stem cells (MSCs) in treating atopic dermatitis. It seeks to determine the efficacy of these EV in modulating cytokine responses, specifically targeting IL-2, IL-4, IL-5, IL-6, IL-10, IL13, and IL-31, which are crucial in the pathophysiology of cutaneous inflammatory disorders. Materials and Methods: MSCs were cultured from adipose tissue and induced to produce EVs. They were then isolated through ultracentrifugation, characterized for particle size and morphology, and encapsulated within calcium alginate nanogels. The anti-inflammatory activity was assessed in vitro using human keratinocyte cultures primed with IFN-gamma and treated with both free and nanoencapsulated EV. The expression of inflammatory cytokines was analyzed through RTq-PCR and protein ELISA Multiplex (Luminex®). Results: The study found that both free and nanoencapsulated EV significantly reduced the expression of IL-2, IL-4, IL-5, IL6, IL-10, IL-13, and IL-31 in IFN-gamma primed keratinocytes. Nanoencapsulation with alginate notably enhanced the delivery efficiency, targeted release, and stability of the EV, leading to a more pronounced reduction in cytokine expression compared to free EV. Conclusion: Nanoencapsulated EV derived from canine MSCs demonstrate significant anti-inflammatory potential, offering a promising therapeutic strategy for atopic dermatitis and other inflammatory conditions. This approach not only preserves the bioactivity of EV but also improves their therapeutic applicability through enhanced delivery and stability. The findings underscore the potential of nanoencapsulation technology in advancing novel therapeutic strategies across human and veterinary medicine, highlighting the importance of cross-disciplinary research in developing effective healthcare solutions.</p> Juliana da Matta Furniel Dutra Fabio Moyses Lins Dantas Caroline Pinho Winck Anael Viana Pinto Alberto Debora Vieira Way Adriana Cristina Motta Elba dos Santos de Oliveira Maria Lucila d’Ottaviano Nápole Juliano C da Silveira Edson Guimarães Lo Turco Copyright (c) 2024 Juliana da Matta Furniel Dutra, Fabio Moyses Lins Dantas, Caroline Pinho Winck, Anael Viana Pinto Alberto, Debora Vieira Way, Adriana Cristina Motta, Elba dos Santos de Oliveira, Maria Lucila d’Ottaviano Nápole, Juliano C da Silveira, Edson Guimarães Lo Turco https://creativecommons.org/licenses/by-nc/4.0 2024-12-05 2024-12-05 8 1 1 17 10.21467/anr.8.1.1-17 https://journals.aijr.org/index.php/anr/article/view/9396 <p>The study is on the green synthesis of zinc oxide nanoparticles and to determine its antibacterial properties as zinc oxide has characteristic physical and chemical properties. The reducing agent green chillie is used to prepare zinc oxide nanoparticles by eco-friendly methods at room temperature. Characterization studies indicated the ZnO nanoparticles were in the range of 67 to 92nm. SEM analysis indicated spherical and cylindrical shaped nanoparticles. Reducing and stabilization characteristics were identified by the presence of functional groups through Fourier Transform Infrared Spectroscopy (FT-IR) analysis. Color change established the synthesis of nanoparticles using the reducing agent of green chili extract. Antibacterial characteristics of the prepared nanoparticles were determined by measuring the zone of inhibition, and good results were obtained.</p> Liny P Jagadish H Patil Copyright (c) 2024 Liny P, Jagadish H Patil https://creativecommons.org/licenses/by-nc/4.0 2025-02-04 2025-02-04 8 1 18 24 10.21467/anr.8.1.18-24